Source: Recobinant. An E. coli strain that carries the cloned ScrFI gene from Streptococcus cremoris F

5′-C C N G G-3′
3′-G G N C C-5′

Reaction Temperature: 37°C

Inactivation Temperature (20 min):  65°C

Prototype: ScrFI

Isoschizomers: MspR9I, Bme1390I, BmrFI

Storage Conditions: Store at -20°C

Unit Definition:
One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hr. Total reaction volume is 50 µl. Enzyme activity was determined in the recommended reaction buffer.

Note:
ScrFI may exhibit star activity when too many excess units are used or if digestion takes too long.

Reaction Buffer:
1x ONE Buffer.
To be supplemented with 100 µg/ml bovine serum albumin.

Storage Buffer:
10 mM Tris-HCl (pH 7.5 at 22°C), 250 mM NaCl, 1 mM dithiothreiol, 0,1 mM EDTA, 0,1% (v/v) Triton™X-100, 200 µg/ml bovine serum albumin and 50 %(v/v) glycerol.

Quality Control:
All preparations are assayed for contaminating endonuclease, 3’exonuclease, as well as nonspecific single- and double-stranded DNase activities.