tris(hydroksymetylo)aminometan
physical form: cristalline powder
purity: molecular biology grade
linear formula: C4H11NO3
molecular weight: 121,14 g/mol
CAS: 77-86-1
An organic compound that, due to its properties, finds wide application in molecular biology and biochemistry.
The presence of both an amine group (–NH₂) and three hydroxymethyl groups (–CH₂OH) in the TRIS structure makes it an effective buffer, ensuring a stable pH in the reaction environment.
The interaction of TRIS with Mg²⁺, Ca²⁺, and Zn²⁺ ions regulates the activity of DNA polymerases, nucleases, and proteases, therefore, it is used as a substrate for buffers such as TRIS-HCl, TBE, TAE, TG, and TE.
