6 x Loading Buffer ORANGE is designated for preparation DNA samples for loading on agarose and polyacrylamide gels. The glycerol content increases the density of the sample and ensures that DNA form a layer at the bottom of the well. EDTA is known for inhibition of metal dependent nucleases. Tracking dyes permit monitoring of progress of electrophoresis.
1% agarose gel (E0301-500) in 1xTBE with SimplySafeTM (E4600)
1—Loading Buffer Blue (E0260) |