SG qPCR Master Mix (2x) is a universal solution for quantitative real-time PCR and two-step real-time RT-PCR and can be used on most real-time PCR cyclers available. The master mix contains Perpetual Taq DNA Polymerase, optimized reaction buffer, dNTPs (dTTP is partially replaced with dUTP) and SYBR Green I dye. SYBR Green I enables DNA analysis without having to synthesize expensive sequence-specific labeled probes.
SYBR Green I is a fluorescent dye which binds all double-stranded DNA molecules and emits a fluorescent signal on binding. The excitation and emission maxima of SYBR Green I are at 494 nm and 521 nm, respectively, which are compatible with use on any real-time cycler.
SG qPCR Master Mix (2x) contains dUTP, which partially replaces dTTP. It allows the optional use of a uracil-N-glycosylase (UNG) to prevent carryover contamination between reactions. UNG removes uracil from any dU-containing contaminating amplicons, leaving abasic sites and making DNA molecules susceptible to hydrolysis during the initial denaturation step.