Fast SG/ROX qPCR Master Mix (2x) is a universal solution for fast-cycling quantitative real-time PCR and two-step real-time RT-PCR and can be used on most real-time PCR cyclers available. The master mix contains Perpetual Taq DNA Polymerase, optimized reaction buffer, dNTPs (dTTP is partially replaced with dUTP) and SYBR Green I dye and ROX. SYBR Green I enables DNA analysis without having to synthesize expensive sequence-specific labeled probes. ROX compensates for variations of fluorescent signal between wells due to slight differences in reaction volume and fluorescence fluctuations. ROX is not involved in PCR reaction.
Fast SG/ROX qPCR Master Mix (2x)
cat. no. E0413