Fast SG/ROX qPCR Master Mix (2x) is a universal solution for fast-cycling quantitative real-time PCR and two-step real-time RT-PCR and can be used on most real-time PCR cyclers available. The master mix contains Perpetual Taq DNA Polymerase, optimized reaction buffer, dNTPs (dTTP is partially replaced with dUTP) and SYBR Green I dye and ROX. SYBR Green I enables DNA analysis without having to synthesize expensive sequence-specific labeled probes. ROX compensates for variations of fluorescent signal between wells due to slight differences in reaction volume and fluorescence fluctuations. ROX is not involved in PCR reaction.