Probe qPCR Master Mix (2x) is a universal solution for quantitative real-time PCR and two-step real-time RT-PCR and can be used on most real-time PCR cyclers available. The master mix contains onTaq DNA Polymerase (hot start), optimized reaction buffer, dNTPs (dTTP is partially replaced with dUTP). Use of the “hot start” enzyme prevents extension of misprimed products and primer-dimers during reaction setup leading to higher specificity and sensitivity of PCR reactions. The polymerase enables convenient room temperature reaction setup. Probe qPCR Master Mix (2x) contains dUTP, which partially replaces dTTP. It allows the optional use of a uracil-N-glycosylase (UNG) to prevent carryover contamination between reactions. UNG removes uracil from any dU-containing contaminating amplicons, leaving abasic sites and making DNA molecules susceptible to hydrolysis during the initial denaturation step.