Blue Triplex ASFV Fast qPCR Kit

cat. no. E0475

High-sensitivity, triple-target detection of African Swine Fever Virus (ASFV) DNA in domestic pigs and wild boars. Validated for whole blood, serum, plasma, swabs (oral, nasal, nasopharyngeal), and tissue/organ samples.

Key Features

  • Detects ASFV in individual or pooled samples (validated for up to 10; recommended ≤5).

  • Compatible with standard or fast real-time PCR programs.

  • Targets a highly conserved ASFV p72 gene fragment (FAM channel).

  • Includes two internal controls:

    • Endogenous – porcine ACTB gene (HEX channel) to verify sample quality.

    • Exogenous – synthetic nucleic acid (Cy5 channel) to monitor extraction efficiency and PCR inhibition.

  • Flexible analysis options: two-channel (FAM, HEX) or three-channel (FAM, HEX, Cy5).

Master Mix Advantages

  • Ready-to-use mix with Perpetual Taq hot-start polymerase, dNTPs, primers/probes, ROX dye, and a visibility-enhancing blue dye for easier pipetting.

  • Room-temperature setup; polymerase activates during a short initial denaturation.

Quality Assurance

  • Positive control contains ASFV DNA, porcine ACTB fragment, and Ex-DNA to confirm proper kit performance.

  • Works with standard silica column or magnetic bead DNA extraction kits.

Blue Triplex ASFV Fast qPCR Kit — reliable, fast, and precise ASFV detection for veterinary diagnostics.