SG OneStep RT-qPCR kit is one-step RT-qPCR kit that provides accurate real-time quantification of RNA targets. Kit is composed of unique reverse transcriptase and highly processive hot start onTaq DNA Polymerase in easy to use format.
The use of ROX passive reference dye is necessary for all real-time PCR cyclers from Applied Biosystems and optional for cyclers from Stratagene. ROX compensates for variations of fluorescent signal between wells due to slight differences in reaction volume and fluorescence fluctuations. ROX is not involved in PCR reaction, has a different emission spectrum than SYBR Green I and does not interfere with real-time PCR on any instrument.