T7 transcription kit with modified T7 RNA Polymerase for higher tolerance towards modified nucleotides. Extremely useful for radioactive and nonradioactive labeling as well as for RNA synthesis in preparative scale. T7 RNA Polymerase is DNA-dependent RNA polymerase with stringent specificity for T7 phage promoters sequence. Efficiently synthesizes transcripts from almost any DNA target located downstream from a T7 promoter. Suitable for preparing labeled single-stranded RNA probes of high specific activity. Transcripts can be used as hybridization probes, templates for in vitro translation, substrates in RNA processing systems, as well as for exon and intron mapping of genomic DNA.