Source: Acinetobacter calcoaceticus
5’-G T M K A C-3’
3’-C A K M T G-5’
Reaction Temperature: 37°C
Inactivation Temperature (20 min): 80°C
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr. Total reaction volume is 50 µl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.