Source: Brevibacterium albidum

5’-T G G C C A-3’
3’-A C C G G T-5’

Reaction Temperature: 37°C

Prototype: BalI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr. Total reaction volume is 30 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, and nonspecific single- and double-stranded DNase activities.