Source: Brevibacterium albidum
5’-T G G C C A-3’
3’-A C C G G T-5’
Reaction Temperature: 37°C
Inactivation Temperature (20 min): 65°C
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr. Total reaction volume is 30 µl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, and nonspecific single- and double-stranded DNase activities.