Source: Flavobacterium okeanokoites
5′-G G A T G (N)9–3′
3’-C C T A C (N)13–5’
Reaction Temperature: 37°C
Prototype: FokI
Inactivation Temperature (20 min): 65°C
Reaction Buffer:
1x ONE Buffer
It is not recommended to use more than 1 unit per 1 μg of DNA and incubate longer than 2 hours.
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 μl.
Storage Conditions: Store at –20°C.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.
