FokI

cat. no. E2170

Source: Flavobacterium okeanokoites

5′-G G A T G (N)93′
3’-C C T A C (N)13–5’

Reaction Temperature: 37°C

Prototype: FokI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

It is not recommended to use more than 1 unit per 1 μg of DNA and incubate longer than 2 hours.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 μl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.