Source: Haemophilus parainfluenzae

5′-C C G G-3′
3′-G G C C-5′

Reaction Temperature: 37°C

Prototype: HpaII

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 μl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities. Ligation – recut assay verified proper enzyme performance.