5′-A C N G T-3′
3′-T G N C A-5′
Reaction Temperature: 37°C
Inactivation Temperature (20 min): 65°C
Prototype: Tsp4CI
Isoschizomers: HpyCH4III, TaaI, Bst4CI
Source: Recombinant. Purified from E. coli strain carrying the cloned HpySP4III from Helicobacter pylori sp. P4.
Package Contents:
- HpySP4III
- 10 x ONE Buffer
- BSA [100x]
Added as separate component to prevent reaction buffer precipitation.
Storage Conditions: Store at -20°C.
Double Digestion – Buffer Compatibility:
ONE Buffer is compatible with most EURx restriction enzymes.
Restriction Enzyme Buffer Compatibility:
Both, enzyme and buffers are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. To obtain best results, consult the corresponding manuals of all involved products.
DNA Methylation:
No Inhibition: dam, dcm, CpG
Notes:
- Overdigestion more than 4 hours with over 5 u per µg DNA of enzyme is not recommended.
- As a result of HpySP4III DNA digestion single 3′ extensions are produced, which are difficult to ligate.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5’ exonuclease/5′-phosphatase, as well as nonspecific single- and double-stranded DNase activities.
