Source: Moraxella bovis
5’-G A A G A (N)8-3’
3’-C T T C T (N)7 -5’
Reaction Temperature: 37°C
Prototype: MboII
Inactivation Temperature (20 min): 65°C
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hr in a total reaction volume of 50 μl.
Storage Conditions: Store at –20°C.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.
