Source: Nocardia otitidis-caviarum

5’-G C G G C C G C-3’
3’-C G C C G G C G-5’

Purified from an E.coli strain carrying the cloned and purified NotI gene from Nocardia otitidis-caviarum.

Reaction Temperature: 37°C

Prototype: NotI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of pBC4 DNA in 1 hr. Total reaction volume is 50 μl. Enzyme activity was determined in the recommended reaction buffer.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, for nickase as well as for nonspecific single- and double-stranded DNase activities. Ligation / recut assays passed successfully.