Source: Nocardia rubra

5’-T C G C G A-3’
3’-A G C G C T-5’

Reaction Temperature: 37°C

Prototype: NruI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hr in a total reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities. Ligation/recut assays passed successfully.