Source: Pseudomonas inaequalis

5’-A C C G G T-3’
3’-T G G C C A-5’

Reaction Temperature: 37°C

Prototype: Age I

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, for nickase as well as for nonspecific single- and double-stranded DNase activities. Ligation / recut assays passed successfully.