Source: Recombinant. Purified from E. coli strain carrying the cloned SfiI gene from Streptomyces fimbriatus

5’-G G C C N N N N N G G C C-3’
3’-C C G G N N N N N C C G G-5’

Reaction Temperature: 37°C

Prototype: SfiI

Inactivation Temperature (20 min): —

Reaction Buffer:

1x ONE Buffer

Unit Definition:
One unit is the amount of enzyme required to complete 98% digestion of 1 µg of pXba in 1 hr. Total reaction volume is 50 µl. Enzyme activity was determined in the recommended reaction buffer.

Storage Conditions: Store at –20°C.

Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease,           5′-exonuclease/5′-phosphatase, as well as nonspecific single- and double-stranded DNase activities.