Native, thermostable DNA polymerase, suitable for applications requiring high temperature synthesis of DNA. Taq DNA Polymeraseis is purified from Thermus aquaticus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C. Catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction in the presence of magnesium ions. Maintains the 5’→3′ exonuclease activity. Lacks the 3’→5′ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb. Native Taq DNA Polymerase is recommended for use in special PCR applications, where traces of E.coli genomic DNA may interfere with amplification specificity.