Thermostable, recombinant thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA. The enzyme is supplemented with two inert gel tracking dyes. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C. Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions. Maintains the 5´->3´ exonuclease activity. Lacks the 3´->5´ exonuclease activity. Adds extra A at the 3′ ends. Color Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
Use of Color Taq DNA Polymerase offers several advantages: visualizes the addition of the polymerase to the reaction, confirms complete mixing, enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer. The added dyes allow to track electrophoresis progress, do not affect PCR performance, do not interfere with most downstream applications (exeption: the polymerase is not recommended for any downstream application using absorbance or fluorescence excitation).