Color Taq DNA Polymerase

cat. no. E2510

Thermostable, recombinant thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA. The enzyme is supplemented with two inert gel tracking dyes. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C. Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions. Maintains the 5´->3´ exonuclease activity. Lacks the 3´->5´ exonuclease activity. Adds extra A at the 3′ ends. Color Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.

Use of Color Taq DNA Polymerase offers several advantages: visualizes the addition of the polymerase to the reaction, confirms complete mixing, enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer. The added dyes allow to track electrophoresis progress, do not affect PCR performance, do not interfere with most downstream applications (exeption: the polymerase is not recommended for any downstream application using absorbance or fluorescence excitation).