Perpetual OptiTaq DNA Polymerase is a modified and balanced blend containing top qualityThermus aquaticus DNA Polymerase, Pyrococcus furiosus DNA Polymerase, anti-TaqDNA Polymerase antibodies, reversible inhibitors and enhancers. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 92-95°C for two minutes. Formation of inactive complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic “hot start” PCR, which allows for the assembly of PCR reactions at room temperature. High stability of the complexes allows for the enormous increase of PCR specificity, sensitivity and yield in comparison to the conventional PCR assembly method. Automatic “hot start” PCR is a convenient method when assembling multiple PCR reactions, saving time and reagents. Enables increased amplification product yield in comparison with Taq DNA polymerase over wide range of PCR products. Maintains the 5’´->3´ exonuclease activity. Adds extra A at the 3′ ends.
Suitable for multiplex PCR due to increased specificity, wider tolerance for Mg2+, salts concentration, and pH. Improves PCR results with critical templates, such as containing GC-rich regions, palindromes or multiple repeats.
Perpetual OptiTaq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 20 kb.