GeneMAGNET Blood DNA Purification Kit is designed for rapid manual or automatic purification of total DNA (genomic, mitochondrial) from fresh or frozen human blood treated with EDTA or sodium citrate anticoagulant using silica-covered magnetic beads.
The new lysis buffer is specially designed to efficiently lyse blood cells, release DNA from nucelic and bind to magnetic beads without contaminants. Traces of contaminants remaining in the solution are efficiently removed in three wash steps. High-quality cellular DNA is then eluted to the DNA storage buffer. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.
|Fig 1. PCR amplification of 1.1 kb, 3 kb and 23 kb DNA fragments from the isolate purified from human blood with GeneMAGNET Blood DNA Purification Kit. Polymerases: Taq PCR Master Mix (2x) E2520 (for 1.1 kb and 3 kb) and Amplus DNA Polymerase EK2900 (for 23 kb fragment). Ctrl-: PCR negative control. DNA on the gel: 57.5 ng/μl, purity A260/A280: 1.825, A260/A230: 1.855, amount 8 μl. 0.7 % agarose 1x TBE.|
Fig 2. Highly efficient qPCR with DNA template purified with GeneMAGNET Blood DNA Purification Kit from the blood sample collected on sodium citrate (green) and EDTA (pink). DNA amount 0.001 ng – 80 ng per 20 μl reaction volume.
|Fig 3. Successful qPCR with even as small DNA amount as 0.01 ng purified with GeneMAGNET Blood DNA Purification Kit. Amplicon length 92 bp. qPCR with Fast SG qPCR Master Mix (2x) E0411, reaction volume 20 μl amount of DNA per reaction: 0.01-10 ng.|
GeneMAGNET line is based on the use of silica paramagnetic beads (Magnetic Beads) for selective binding of RNA and DNA. The use of specially designed binding and washing buffers enables the efficient purification of highly pure nucleic acids.