GeneMATRIX Agrobacterium Plasmid Miniprep DNA Purification Kit is designed to isolate high purity plasmid DNA from soil bacteria Agrobacterium tumefaciens. Plasmid DNA contaminants such as: RNA, singlestranded DNA, enzymes/proteins, lipids, dyes, detergents, nucleotides, EDTA, problematic restriction and ligation inhibitors, buffers and salts are effectively removed from crude bacterial lysate. The procedure is especially optimized toward isolation of palsmids larger than 10 kb from Agrobacterium species. The addition of the lysozyme incubation (with BL buffer) improves lysis and increases plasmid yield. Coloured lysis buffer helps both in monitoring cell solubilization progress as well as simultaneous processing of multiple samples. Optimized buffer is added to provide selective conditions for DNA binding to the GeneMATRIX during brief centrifugation, while contaminants pass through the spin-column. Traces of contaminants remaining on the membrane are efficiently removed in two wash steps. Highquality DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.