GeneMATRIX Basic DNA Purification Kit is a useful basic tool in any laboratory working with DNA. It allows to perform three basic laboratory techniques: DNA purification after enzymatic treatment, isolation of DNA from agarose gels and the isolation of plasmid DNA from bacterial cultures. Combining these capabilities kit allows to streamlin work and minimizing the costs of research projects.
PCR / DNA Clean-Up Protocol allows for purification of DNA fragments, which were subjected to or obtained as a result of various modifications and reactions: PCR products, restriction digests, after kinasing, dephosphorylation, end-trimming/repair, ligation, enzymatic or chemical modification, among others. Fragment of sizes from approximately 100 bp to over 15 kb can be obtained in ultrapure form. Effectively removed are contaminants such as: ethidum bromide, primers (below 40 nt), short double-stranded DNA (below 20 bp), RNA, Taq DNA Polymerase, Pfu DNA Polymerase, endo- and exonucleases, DNA-binding and modifying proteins, BSA and other enzymes/proteins, lipids, endotoxins, dyes, detergents, nucleotides, radio- and chemical labels, EDTA, problematic restriction and ligation inhibitors, buffers and salts.
Agarose-Out Protocol is designed to isolate linear or circular DNA molecules, ranging in size from approximately 100 bp to 10 kb, from TAE- or TBE-agarose gels. It is also possible to purify DNA fragments up to 20 kb or more, with gradually decreasing yields. Besides agarose many other contaminants are effectively removed: ethidium bromide, RNA, primers, enzymes and other proteins, lipids, endotoxins, dyes, detergents, nucleotides, radio- and chemical labels, EDTA, problematic restriction and ligation inhibitors, buffers and salts.
Plasmid Isolation Protocol allows for purification of high purity plasmid DNA from various species of bacteria, including recombinant Escherichia coli strains. Plasmid DNA contaminants such as: RNA, single-stranded DNA, enzymes/proteins, lipids, dyes, detergents, nucleotides, EDTA, problematic restriction and ligation inhibitors, buffers and salts are effectively removed from crude bacterial lysate. Coloured lysis buffer helps both in monitoring cell solubilization progress as well as simultaneous processing of multiple samples.
For all protocols optimized buffer is added to provide selective conditions for DNA binding to the GeneMATRIX membranes during brief centrifugation, while contaminants pass through the spin-column. Traces of contaminants remaining on the membrane are efficiently removed in two wash steps. High-quality DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.
