Bone DNA Purification Kit

cat. no. E3560

GeneMATRIX Bone DNA Purification Kit is designed for rapid purification of DNA (genomic, mitochondrial) from animal or human bones and teeth. Purified DNA is free of contaminants, such as: proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
A bone sample is finely grinded and the obtained powder is subsequently solubilized by lysis in the presence of special desintegrating buffer, which preserves and stimulates quantitative recovery of all traces of DNA. Further, Proteinase K digests collagen and other proteins. Optimized buffer and ethanol are added to provide selective conditions for DNA binding during brief centrifugation, while contaminants pass through the GeneMATRIX resin in the spin-column. Traces of contaminants remaining on the resin are efficiently removed in two wash steps. High-quality DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.