GeneMATRIX Plant & Fungi DNA Purification Kitis designed for rapid purification of total DNA (genomic, mitochondrial and chloroplast) from a wide variety of plant, fungi and lichenes tissues. Purified DNA is free of contaminants, such as: RNA, proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
Sample is finely grinded and remaining tissue- and cellular structures are subsequently solubilized by lysis in the presence of special desintegrating buffer, which preserves integrity and stimulates quantitative recovery of all traces of DNA. Further, Proteinase K digests contaminating proteins, including stripping-off DNA of all bound proteins, among them nucleases. Optimized buffer and ethanol are added to provide selective conditions for DNA binding during brief centrifugation, while contaminants pass through the GeneMATRIX resin in the spin-column. Traces of contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.