The GeneMATRIX Universal RNA Purification Kit is designed for rapid, thorough isolation and purification of total RNA from a broad variety of sources: animal, plant, fungi tissues, cell cultures, bacteria and yeast cells or blood (leukocytes), among others.
Samples are first lysed in the presence of a denaturing buffer. Upon lysis buffer addition, cellular RNases are immediately inactivated. A passage through specifically designed homogenization spin‑columns results in shearing of genomic DNA, thus reducing viscosity of the lysate and removing DNA fragments. The flow‑through is applied to a binding spin‑column with high specifity for RNA. RNA molecules are adsorbed to the matrix and contaminants are efficiently washed away. High‑quality RNA is then eluted in RNase‑free water. Typical yields are up to 125 μg total RNA longer than 200 bases. However, it is possible to purify RNA molecules smaller than 200 bases, with gradually decreasing efficiency. Isolated RNA is ready for downstream applications without the need for ethanol precipitation.
Purified RNA is of high quality, is DNA‑free as well as free of contaminants, such as DNA, proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others. Rock‑solid GeneMATRIX technology forms the basis for efficient recovery of pure RNA.