The XELEX Kit series is a modular kit system covering the entire SELEX work-flow from random DNA library to RNA aptamer. This kit presents an optimized, straightforward standard operating procedure for the whole SELEX process. It consists of a core kit and several add-on packages which add functionality for individual adjustment of the selection process to experimental requirements.
The kit is subdivided into two units, a selection unit and an analysis unit. Whereas the selection unit covers the entire range of the actual SELEX enrichment and aptamer selection procedure from random DNA libraries, the analysis unit deals with all issues related to quality control and initial characterization of both, the enriched libraries as well as of those aptamers identified as good binders.
Novel approaches help to significantly cut down hands-on time. SELEX is no longer a matter of several months, now it is a matter of weeks. The overall kit design is optimized towards maximum experimenters flexibility. For critical steps within this procedure, outlines for alternate approaches are given. This ensures compatibility of the kit with high end technologies such as semi-automated selections and Next Generation Sequencing, as well as enabling the researcher to conduct SELEX reactions by using no more than standard laboratory equipment.
Depending on the nature of the selection target, the experimenter is free to choose the most gentle and appropriate strategy from different target immobilization methods. Undecided whether DNA or RNA aptamers suit your needs best? No problem, simply perform protocols for DNA and RNA aptamer selections in parallel. Runoff amplification of non-specific but preferentially amplified molecules is efficiently prevented by proven technology of our Micellula emulsion kits. Our rock-solid GeneMatrix technology warrants efficient purification of DNA and RNA aptamers.
Valuable add-ons extend the functionality of the XELEX kit and add specific modifications for fine adjustment of the selection process to experimental requirements. These add-ons include error-prone random mutagenesis amplifications (for introduction of additional diversity during selection cycles), or for Sortase-mediated protein immobilization under physiological conditions (for sensitive target proteins).