AnEx Plasmid Midiprep DNA Purification Kit is designed to isolate ultrapure plasmid DNA from various species of bacteria, including recombinant Escherichia coli strains.
Plasmid DNA contaminants such as: RNA, single-stranded DNA, enzymes/proteins, lipids, dyes, detergents, nucleotides, EDTA, problematic restriction and ligation inhibitors are effectively removed from crude bacterial lysate. The anion exchange resin used for construction of the column is especially optimized toward removal of problematic inhibitors of restriction of DNA as well as non-specific endo- and exonucleases. Coloured lysis buffer helps in monitoring cell solubilization progress. Optimized low salt buffer provide selective conditions for binding plasmid DNA to the resin. During gravity flow contaminants pass through the column and traces of contaminants remaining on the resin are efficiently removed in wash step. High-quality plasmid DNA is then eluted in high salt buffer. After precipitation isolated DNA is ready for downstream application.