Anchored Oligo(dT)20 VN Primer is a primer mixture consisting of a string of 20 thymidyne residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC) at the 3’end. Due to the variable end-sequence, the primer is anchored to the 5 ́-end of the poly(A) tail of mRNA and prevents priming within the poly(A) tail.
Anchored Oligo(dT)20 VN primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA and is recommended for use in cDNA labeling protocols. Primer is supplied in DEPC-treated water at aconcentration of 50 μM (0.3μg/μl).
