Blank qPCR Master Mix (2x)

cat. no. E0403

Blank qPCR Master Mix (2x) is a universal solution for quantitative real-time PCR and two-step real- time RT-PCR and can be used on most real-time PCR cyclers available. The master mix does not contain any dye and allows the user to choose a dye and optimize the dye concentration. The master mix contains Perpetual Taq DNA Polymerase, optimized reaction buffer and dNTPs (dTTP is partially replaced with dUTP).

Blank qPCR Master Mix (2x) contains dUTP, which partially replaces dTTP. It allows the optional use of a uracil-N-glycosylase (UNG) to prevent carryover contamination between reactions. UNG removes uracil from any dU-containing contaminating amplicons, leaving abasic sites and making DNA molecules susceptible to hydrolysis during the initial denaturation step.