Blue SG onTaq qPCR Master Mix (2x) is a universal solution for quantitative real-time PCR and two-step real-time RT-PCR built around the modified onTaq hot-start DNA Polymerase, activated by a 15-minute initial denaturation at 95°C. It pairs SYBR Green I detection with a non-reactive blue tracking dye for easy pipetting control, and its dUTP/dTTP composition supports optional uracil-N-glycosylase (UNG) treatment against carryover contamination. This catalog number is the variant supplied without ROX, suited to instruments that do not require a passive reference dye.