SG OneStep RT-qPCR Kit is one-step RT-qPCR kit that provides accurate real-time quantification of RNA targets. Kit is composed of unique reverse transcriptase and highly processive hot start onTaq DNA Polymerase in easy to use format. During reverse transcription step at 50°C thermolabile uracil-N-glycosylase can be used. Do not use UNG from E.coli, which will degrade all newly synthetized cDNA.