Direct Tissue PCR Kit

cat. no. E0940

Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no prior DNA purification.

Description:

  • The Direct Tissue PCR Kit allows to use samples such as: animal tisues, mouse ear and tail, zebrafish fin, Drosophila, human hair, saliva and other liquid samples.
  • The samples can either be fresh or stored at -20°C.
  • The Direct Tissue PCR Kit employs a hot start and genetically engineered thermophilic DNA polymerase with high tolerance to blood inhibitors.
  • Tissue DNA Polymerase activity is restored during 7 min initial denaturation step.
  • Tissue DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction.
  • The enzyme exhibits the 3’→5′ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases.
  • The enzyme generates blunt ends.
  • Enhanced polymerase processivity allows to use shorter extension times.
  • Due to the genetic modification of the polymerase, the optimal reaction conditions (especially annealing temperatures) differ from standard PCR protocols.
  • The Direct Tissue PCR Kit contains reagents for two alternative protocols: Direct and Extract protocols.
  • The master mix contains premixed gel loading reagent and dyes which allow direct sample loading on the gel.
  • The Direct Tissue PCR Kit allows to obtain a wide range of product size (over 4 kb).

Direct Tissue PCR Kit contains:

  1. 2 x Tissue PCR Master Mix
  2. Tissue DNA Polymerase
  3. Extraction Buffer
  4. Lysis Enhancer
  5. Water, nuclease free