Direct Plant PCR Kit

cat. no. E0960

Direct Plant PCR Kit enables to perform PCR reactions directly from plant samples with no prior DNA purification.

Description:

  • The Direct Plant PCR Kit allows to use samples such as: plant leaves , plant seeds and plant material stored on commercially available cards.
  • The samples can either be fresh or stored at -20°C.
  • The Direct Plant PCR Kit employs a hot start and genetically engineered thermophilic DNA polymerase with high tolerance to plant inhibitors.
  • DNA Polymerase activity is restored during 10 min initial denaturation step.
  • DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction.
  • The enzyme exhibits the 3’→5′ proofreading activity, resulting in 50-fold higher PCR fidelity than possible with Taq DNA Polymerases.
  • The enzyme generates blunt ends.
  • Enhanced polymerase processivity allows to use shorter extension times.
  • Due to the genetic modification of the polymerase, the optimal reaction conditions (especially annealing temperatures) differ from standard PCR protocols.
  • The Direct Plant PCR Kit contains reagents for two alternative protocols: Direct and Extract protocols.
  • The master mix contains premixed gel loading reagent and dyes which allow direct sample loading on the gel.
  • The Direct Plant PCR Kit allows to obtain a wide range of product size (over 4 kb).

Direct Plant PCR Kit contains:

  1. 1. 2 x Plant PCR Master Mix
  2. DNA Polymerase
  3. Extraction Solution
  4. Dilution Solution
  5. Water, nuclease free

2 x Plant PCR Master Mix:
The master mix contains 2 x concentrated optimized PCR buffer, 5 mM MgCl2, dNTPs and two gel tracking dyes.

DNA Polymerase Storage Buffer:
20 mM Tris-HCl (pH 9.0 at 22°C), 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 50% glycerol and stabilizers.

Quality Control:
All preparations are assayed for contaminating 3′-exonuclease, and nonspecific single – and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.