Alkaline Phosphatase is calf intestine derived phosphatase which catalyzes the hydrolysis of phosphate monoesters. Has higher turnover rate and milder conditions of inactivation than bacterial enzyme. Can be used to remove 5′-phosphates from DNA or RNA prior to 5′-end labeling. Used to remove 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during […]

Endocyclase N15 (Protelomerase N15) catalyzes formation of covalent links between complementary 5′- and 3′- ends (clamps DNA strands together) and forms exonuclease-protected hairpin ends at specific double-stranded DNA recognition sites. Generates covalent clamps between two complementary DNA strands on one or on both ends. Converts PCR products to linear plasmids with capped “hairpin” ends. Converts […]

Exo-BAP Mix is used for enzymatic PCR cleanup prior to sequencing and Single Nucleotide Polymorphism (SNP) analysis. It allows quick and effective removing of any unconsumed dNTPs and primers remaining in the PCR product mixture. It contains two selected enzymes, thermosensitive bacterial alkaline phosphatase and Exonuclease I in specially composed storage buffer.  

Thermosensitive Bacterial Alkaline Phosphatase catalyzes the release of 5′- and 3′-phosphate groups from DNA, RNA, and nucleotides. Removes 5´ phosphates from DNA, RNA, rNTPs and dNTPs. Removes 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. The enzyme is resistant to chemical changes and active over a broad range of […]

Thermosensitive Bacterial Alkaline Phosphatase catalyzes the release of 5′- and 3′-phosphate groups from DNA, RNA, and nucleotides. Removes 5´ phosphates from DNA, RNA, rNTPs and dNTPs. Removes 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. The enzyme is resistant to chemical changes and active over a broad range of […]

Rec A Protein is a bacterial recombination protein that enhances interaction and exchange of homologous DNA strands. It regulates repair mechanisms and repairs DNA. Involved in DNA renaturation and rare cleavage of genomic DNA. Binds cooperatively and stechiometrically to single-stranded DNA. In presence of ATP-γ-S, locates and pairs a single-stranded DNA sequence to its homologous […]