Alkaline Phosphatase is calf intestine derived phosphatase which catalyzes the hydrolysis of phosphate monoesters. Has higher turnover rate and milder conditions of inactivation than bacterial enzyme. Can be used to remove 5′-phosphates from DNA or RNA prior to 5′-end labeling. Used to remove 5′-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures. Applicable to dephosphorylation of proteins.