DNA Polymerase I is a mezophilic, DNA-dependent DNA polymerase with inherent 3’→5′ and 5’→3′ exonuclease activity. Exhibits the 5’→3′ polymerase activity. Used to prepare radioactive probes by nick translation and random priming. Useful for end-labeling of DNA molecules with 3’ and 5’ protruding tails or blunt-ended.
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Bst DNA Polymerase (Large Fragment, exo-)
cat. no. E1078
Large exonuclease free fragment of thermostable Bst DNA Polymerase with strand displacement activity. Bst DNA Polymerase is a moderately thermostable enzyme from Bacillus stearothermophilus. The enzyme replicates DNA optimally at 65°C. Catalyzes the polymerization of nucleotides into duplex DNA in the 5’→3′ direction in the presence of magnesium ions. Lacks the 5’→3′ exonuclease activity. The […]
Bsu DNA Polymerase
cat. no. E1450
Description: Ideal for strand displacement DNA synthesis. The enzyme replicates DNA optimally at 37°C. Devoided of both 3’→5’ exonuclease the 5’→3’ exonuclease activities, while retaining the polymerase activity. Ultrapure, recombinant enzyme. Suitable for second strand cDNA synthesis. Can be used in random primer labeling and single dA tailing. Can be heat inactivated at 75°C for […]
Klenow Fragment (E.coli)
cat. no. E1091
Klenow Fragment. Large fragment of E. coli DNA Polymerase l enzyme which retains both the polymerase and the proofreading 3’→5′ exonuclease activities of Polymerase l. Lacks the 5′-exonuclease activity. Used for Sanger dideoxy sequencing. Suitable for second strand cDNA synthesis. Used for 3′-end labeling and filling in 5′-protruding sticky ends.
Klenow Fragment (exo-)
cat. no. E1092
Large fragment of E. coli DNA Polymerase l, which lacks both the 3’→5′ exonuclease and the 5’→3′ exonuclease activities of Polymerase l. Used for Sanger dideoxy sequencing. Suitable for second strand cDNA synthesis. Used for 3′-end labeling and filling in 5′-protruding sticky ends.
MDA phi29 Kit
cat. no. E1402
MDA phi 29 Kit contains phi29 DNA Polymerase, that catalyzes a highly processive DNA synthesis (up to 70,000 base insertions per binding event) coupled to strand displacement activity(1). It possesses an inherent 3’-5’ exonuclease activity(2) acting preferentially on single-stranded DNA. The kit is designed to perform MDA (multiple displacement amplification) reactions. The Modified Random Hexamer […]
Opti Bst DNA Polymerase LF
cat. no. E1079
Large exonuclease 5’→3’ free fragment of thermophilic Bst DNA Polymerase with strand displacement activity. Description: Opti Bst DNA Polymerase is a moderately thermostable enzyme from Bacillus stearothermophilus genetically optimized for faster amplification and more flexible reaction conditions. Optimized for LAMP (Loop Mediated Isothermal Amplification) Enhanced dUTP incorporation compared to Bst DNA Polymerase. Ultrapure, recombinant protein. […]
