T5 Exonuclease is a product of T5 phage D15 gene derived from E. coli. T5 exonuclease degrades DNA in a 5’→3′ direction and also exhibits endonucleolytic activity on flap structures (branched duplex DNA containing a free single-stranded 5′-end). T5 exonuclease has been shown to have no effect on double stranded circular DNA.
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DNase I (RNase-free)
cat. no. E1345
DNase I, nonspecific deoxyribonuclease that degrades both double-stranded and single-stranded DNA, endonucleolytically releasing 5′-phosphorylated di-, tri-, and oligonucleotide products. DNase I is used to remove template DNA following in vitro transcription, to remove contaminating DNA in total RNA preparations, to prepare DNA–free RNA prior to RT-PCR and RT-qPCR.
Exonuclease I
cat. no. E1150
Exonuclease I, catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5′ direction. Does not degrade dsDNA or RNA. Active under a wide variety of buffer conditions. Exonuclease I is ideal for: removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis, removal of single-stranded primers for nested PCR reactions, […]
Exonuclease III (E.coli)
cat. no. E1140
Exonuclease III catalyzes the removal of mononucleotides from dsDNA starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases, yielding nucleoside 5´-phosphates. The enzyme contains DNA 3′-phosphatase, hydrolyzing 3′-terminal phosphomonoesters and AP endonuclease, cleaving phosphodiester bonds at apurinic or apyrimidinic sites to produce 5′-termini that are base-free deoxyribose […]
Exoribonuclease XRN-1
cat. no. E1155
XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. Description: XRN-1 also acts on 5´ monophosphate ssDNA with greatly reduced efficiency. XRN-1 is not efficient in removing RNAs with recessed 5´P, like tRNA. It does not cleave dsDNA, ssDNA and RNA which contain di-, triphosphate, OH and capped 5´ ends.
Lambda Exonuclease
cat. no. E1180
Lambda Exonuclease, double-stranded specific DNase produced by Escherichia coli upon lambda bacteriophage infection. Lambda exonuclease processively degrades one strand of double-stranded DNA (dsDNA) in the 5’→3′ direction. The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA. Lambda exonuclease has reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini.
OMNI Nuclease
cat. no. E1120
OMNI Nuclease catalyzes the removal of all forms of DNA and RNA (linear, circular, double and single stranded). Nuclease completely degrades nucleic acids to 5′-monophosphorate terminated oligonucleotides of 2 – 6 in length. Applications: viscosity reduction in bacterial, yeast and mammalian protein extracts, sample preparation for protein 2D electrophoresis, removal of nucleic acids contaminants from […]
