Exonuclease I, catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5′ direction. Does not degrade dsDNA or RNA. Active under a wide variety of buffer conditions. Exonuclease I is ideal for: removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis, removal of single-stranded primers for nested PCR reactions, removal of ssDNA from dsDNA.