Thermus thermopilus Reverse Transcriptase catalyzes the reverse transcription of RNA to cDNA at elevated temperatures in the presence of Mn²⁺ and catalyzes polymerization of DNA in the presence of Mg²⁺.

• Suitable for high temperature synthesis of DNA.
• Synthesizes cDNA from RNA template.
• Minimizes problems with strong secondary structure of RNA.
• Used for efficient PCR of DNA, containing problematic secondary structures.
• Applicable to RT-PCR. The same enzyme is used for both reverse transcription and following amplification of obtained cDNA template.
• Resistant to amplification inhibitors present in template DNA isolated from problematic samples.