Source: Bacillus stearothermophilus HKC

5’-C Py C G Pu G-3’
3’-G Pu G C Py C-5’

Note 1: Purified from E.coli strain that carries the cloned bsiHKCRI gene.

Reaction Temperature: 65°C

Prototype: AvaI

Inactivation Temperature (20 min): —

Reaction Buffer:

1x ONE Buffer

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.