Source: Bacillus stearothermophilus HKC
5’-C Py C G Pu G-3’
3’-G Pu G C Py C-5’
Note 1: Purified from E.coli strain that carries the cloned bsiHKCRI gene.
Reaction Temperature: 65°C
Inactivation Temperature (20 min): —
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities.