Source: Bacillus stearothermophilus X
5’-C C A N N N N N N T G G-3’
3’-G G T N N N N N N A C C-5’
Reaction Temperature: 50°C
Prototype: BstXI
Inactivation Temperature (20 min): 65°C
Reaction Buffer:
1x High Buffer: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin.
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
Quality Control:
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as nonspecific single- and double-stranded DNase activities.
