cat. no. E2118

Source: Bacillus stearothermophilus X

5’-C C A N N N N N N T G G-3’
3’-G G T N N N N N N A C C-5’

Reaction Temperature: 50°C

Prototype: BstXI

Inactivation Temperature (20 min): 65°C

Reaction Buffer:

1x High Buffer: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, 1 mM dithiothreitol, 100 µg/ml bovine serum albumin.

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.

Storage Conditions: Store at –20°C.

Quality Control:

All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as nonspecific single- and double-stranded DNase activities.