Source: Haemophilus parainfluenzae
5′-C C G G-3′
3′-G G C C-5′
Reaction Temperature: 37°C
Inactivation Temperature (20 min): 65°C
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 μl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, as well as non-specific single- and double-stranded DNase activities. Ligation – recut assay verified proper enzyme performance.