Source: Pseudomonas inaequalis
5’-A C C G G T-3’
3’-T G G C C A-5’
Reaction Temperature: 37°C
Prototype: Age I
Inactivation Temperature (20 min): 65°C
1x ONE Buffer
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hr in a total reaction volume of 50 µl.
Storage Conditions: Store at –20°C.
All preparations are assayed for contaminating endonuclease, 3′-exonuclease, 5′-exonuclease/5′-phosphatase, for nickase as well as for nonspecific single- and double-stranded DNase activities. Ligation / recut assays passed successfully.